Courses

Introduction to mass spectrometry and its use with different hyphenations. Focus on the different ionisation techniques used, together with different analysers. Throughout the construct of the molecule is used to direct the user to the correct ionisation and sample introduction technique(s). The need to understand and use the correct nomenclature and performance criteria of the mass spectrometer and related experiments is also stressed, thus allowing for clear communication of experiments and results. Strategies for data interpretation, together with some simple mechanistic approaches to fragmentation are shown with this linking to basic tandem MS, these include understanding the behaviour of odd electron and even electron species.

Presenter: John Langley

Professor John Langley is an expert in mass spectrometry and chromatography, researching complex systems through the application and development of different analytical approaches. John has over 130 peer-reviewed publications (more than 150 in Google Scholar) and an H-index of 35 and has published in over 30 leading scientific journals, reflecting the multidisciplinary nature of the applications of his research activity.

John has 40 years of experience in the academic sector and specializes in the application of separation science and mass spectrometry as a research tool for chemistry, chemical biology, and oilfield chemistry, addressing complexity and probing new research areas. Specific interests are the application of hyphenated approaches (GC-MS, GCxGC-MS, HPLC-MS, LCxLC-IMS-MS, MS/MS, SFC-MS and SFC-FID).

John has 30 years of sustained research funding with RCUK and industry, (including AZ, BP, GSK, Innospec, and Syngenta) and was awarded the Chromatographic Society Jubilee Medal 2024 and the British Mass Spectrometry Society Medal 2021.

If you use capillary electrophoresis (CE) in your work and want a better understanding of the technique, or want to start with CE and want to be well prepared, this course is for you. The course is designed for analytical scientists and technicians who use CE in their regular job, but also lab managers and quality assurance or regulatory people who need to review CE work might find it useful. No previous CE training is assumed, however, much of the course will also appeal to the experienced scientist who wants a firm ground in the basics of CE.

“Introduction to Capillary Electrophoresis” explains the fundamentals of CE from the ground up. The course comprises:

• The basic principles of capillary electrophoresis
• Ten different modes of CE, including CZE, CGE and cIEF
• Equipment and operational details
• Treatment of the capillary, the separation column
• Electrokinetic and hydrodynamic injection
• Multiple detection mechanisms
• Corrected peak areas explained
• How to get started with method development and how to predict the migration order
• Tips for successful implementation of CE in your lab

‘GC-MS for Operators and Method Developers’ is specifically designed for GC users and operators. Fundamental principles, when presented, are done so with a focus on the practical aspects and applications as might be applied on a daily basis.

Those who complete this course should be more efficient and comfortable at setting up, troubleshooting, verifying performance, acquiring and interpreting GC-MS data. As with “Introduction to GC” and “Intermediate GC” courses, the GC-MS course is organized into chapters and modules. The chapters focus on given themes and the modular structure underneath exposes students to subtopics within time-efficient segments.

This course is designed for users of gas chromatographs who are looking to further development their method development and troubleshooting skills in GC-MS.

Presenter: Matthew Klee

Dr Matthew S. Klee is a recognized authority in the area of GC analysis and instrumentation. Armed with a Ph.D. in analytical chemistry and 7 years in the chemical and pharmaceutical industries, he joined HP (later to become Agilent Technologies). During 23 years at HP/Agilent, Dr Klee was involved with the invention and commercialization with many of the GC and GC-MS products that Agilent now sells. He regularly teaches GC short courses.

The Intermediate Gas Chromatography tutorial is designed for GC users who have a fundamental understanding of how gas chromatography works and who are ready to develop a more in-depth understanding of the technique. As with the ‘Introduction to Gas Chromatography’ course, the ‘Intermediate Gas Chromatography’ course is organized into sections and modules. The sections focus on a given theme and the modular structure underneath exposes students to the information within time-effective sections. The ‘Intermediate Gas Chromatography’ course is a logical next step after completing the ‘Introduction to Gas Chromatography’ course.
Upon successful completion of the course, trainees will have increased knowledge and skills in development of gas chromatography methods. They will gain an understanding of both qualitative and quantitative methods, as well as expanding their skills to encompass areas such as fast GC, and further developing maintenance and troubleshooting abilities.

This course is designed for users of gas chromatographs who are looking to further development their method development and troubleshooting skills. A key demographic is companies who provide their employees with yearly skill set development objectives. Even though supported by management, fulfilment of these objectives is difficult to accomplish through traditional training due to scheduling issues and frequent work-related interruptions. The on-line tutorial approach provides a unique solution by being accessible from anywhere with internet access, and at any time.

Presenter: Matthew Klee

Dr Matthew S. Klee is a recognized authority in the area of GC analysis and instrumentation. Armed with a Ph.D. in analytical chemistry and 7 years in the chemical and pharmaceutical industries, he joined HP (later to become Agilent Technologies). During 23 years at HP/Agilent, Dr Klee was involved with the invention and commercialization with many of the GC and GC-MS products that Agilent now sells. He regularly teaches GC short courses.

The ‘Introduction to Gas Chromatography’ On-Line Tutorial is basic introduction to the theory and practice of gas chromatographic analysis. It is designed to bring GC users to a more comfortable and capable level of proficiency in instrument operation, optimization, and troubleshooting. Lessons are presented in a visually engaging and accessible manner through narrated on-line video presentations. In full recognition of the difficulty in setting large blocks of uninterrupted time for training; the lessons are presented in short segments that can be easily fit into even the busiest schedules.

• This course presents the basic processes and instrumentation involved with GC analysis.
• The level of material is specifically designed for GC users without extensive formal training.
• Applicable theory is covered to the extent that it helps guide best practices, method optimization and effective troubleshooting.

Upon successful completion of the tutorial, trainees will have increased confidence and skills in using gas chromatographs. They will better understand how GC works, be more competent at detecting and diagnosing problems, and will be better at interpreting the quality of analytical results. In the end, trainees will be more capable and comfortable using gas chromatographs.

Presenter: Matthew Klee

Dr Matthew S. Klee is a recognized authority in the area of GC analysis and instrumentation. Armed with a Ph.D. in analytical chemistry and 7 years in the chemical and pharmaceutical industries, he joined HP (later to become Agilent Technologies). During 23 years at HP/Agilent, Dr Klee was involved with the invention and commercialization with many of the GC and GC-MS products that Agilent now sells. He regularly teaches GC short courses.

Although “small molecule” and “biopolymer” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with both. Fortunately, the underlying principles of chromatography apply equally well in both situations when interpreted appropriately.

“Practical HPLC for Biopharmaceuticals” explains chromatography in practical terms from the ground up. Here’s what you’ll learn in this online course:

• The basics of isocratic and gradient HPLC
• Reversed phase and ion exchange HPLC
• HPLC equipment – from reservoirs/pumps to tubing/injectors to columns and detectors
• Understanding and overcoming common column problems
• Alternative modes: normal phase, HILIC and size exclusion
• Quantitation
• UHPLC
• Performance qualification
• Troubleshooting and diagnostics

Presenter: Tom Jupille

Tom has been a practising chromatographer for more than 45 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in late ‘70s. His career has focused on instrument and column development and user support, providing a broad foundation of practical experience to call on as an instructor. He is moderator of the popular Chromatography Forum on-line chromatography discussion group.

This class focuses on validating high-performance liquid chromatographic (HPLC) methods developed for the analysis of pharmaceutical drug substance (pure drugs), drug product (formulated drugs), impurities, and degradation products. Although the focus is on pharmaceuticals, the principles apply for most quantitative HPLC methods, such as environmental or general chemical analyses.

Here’s what the course covers:

• What the steps are that need to be undertaken during validation
• Why quality is so important
• What basic chromatographic measurements will be used
• Why to approach validation as part of a larger process
• Which regulatory documents are important
• What parameters of the method need to be tested
• What are the different standardization techniques, and when are they used
• What is Quality by Design, and why is it important
• What software tools are available to simplify the process
• What pitfalls should be avoided in validation
• What is pre-validation, and why is it worth my time
• How do I choose a system suitability sample
• How can I tell if the method is out of control
• How much can I adjust a method before I have to re-validate

Presenter: John Dolan

John Dolan is considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.

With the introduction of the mass spectrometer (MS) as a practical detector for a high-performance liquid chromatograph (LC or HPLC) in the early 1990s, LC-MS began to be used for routine applications. One form of LC-MS uses a tandem MS (LC-MS/MS), that has become the go-to instrument for quantitative analysis of drugs in biological matrices in the pharmaceutical laboratory. As they have become more widely used and understood, LC-MS and LC-MS/MS have found their way into environmental applications, synthetic chemistry, protein analysis, and many other areas. LC-MS/MS has moved from a technique available only to skilled experts in both HPLC and MS to a routine tool usable much as any other HPLC detector.

You will find that this introductory course in LC-MS/MS will give you a good foundation of understanding the technique and how to use it in your daily work. We start out with an overview of different MS techniques, and focus in the majority of the course on the quadrupole mass spectrometer. You will learn how MS works, how ions are produced and fragmented. Sections focus on qualitative, as well as quantitative analysis. Each part of the process, calibration, tuning, sample preparation, HPLC method development, and MS method development are covered. Many tricks and techniques of practical use are presented.

Here’s what the course covers:

• MS operation, including the operation of the most popular LC-MS interfaces
• How a quadrupole mass filter works
• MS calibration and optimization
• Ion production, fragmentation, and detection
• Operation in MS, MS/MS, and MS/MS/MS modes
• Structure determination by product ion analysis
• Quantitative analysis
• Sample preparation
• Method development
• Validation
• Techniques to get the most from your LC-MS

Presenter: John Dolan

John Dolan is considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.

“Advanced HPLC Method Development Using Quality by Design” is an intensive online course that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods. Here’s what the course covers:

• What Quality by Design (QbD) is and how it applies to HPLC methods
• A review of basic HPLC theory as it applies to method development
• The makeup of HPLC columns including the support material and the bonded phase
• The role of solvents in the separation
• How the column plate number, selectivity, and retention can be used in a logical way to solve even the
  toughest separation problems.
• How to use mobile phase solvents, pH, temperature, chemistry, and other variables to pull apart
  difficult-to-separate peak pairs.
• How gradient elution works and how to remove the mystery of optimizing gradients.
• How a scouting gradient can be used to speed the development of both isocratic and gradient methods.
• How to use a simple spreadsheet calculator to move from the scouting run to the next step in method
  development
• How computer-assisted method development tools can leverage a small amount of data into a large amount of information about a separation
• How to take advantage of a publicly available internet database to identify equivalent and orthogonal columns
• When standard reversed-phase techniques don’t work, how to choose mixed-mode, ion-pairing, normal phase, HILIC, chiral, or other chromatographic modes
• What’s the same and different when comparing HPLC and UHPLC
• What to look for when validating a method

Presenter: John Dolan

John Dolan is considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.

“HPLC & UHPLC Troubleshooting: A Performance Qualification Approach” is an intensive online course that teaches you the ins and outs of solving problems that occur with your high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) methods. You will learn how to qualify the performance of your HPLC or UHPLC using specific tests that also can be used for isolating existing problems. More importantly, you’ll learn how to prevent many of these problems from happening in the first place.

This course is designed for anyone who works with HPLC or UHPLC. No previous experience with HPLC or UHPLC systems is assumed; however, much of the course will appeal to the more experienced worker.

Presenter: John Dolan

John Dolan is considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.