“Advanced HPLC Method Development Using Quality by Design” is an intensive online course that teaches you strategy and technique to develop high-quality HPLC and UHPLC methods quickly and with confidence. You will learn a time-proven technique that walks you through the method development process a step at a time using sound chromatographic principles to help make the process more efficient. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. Because the same principles apply to HPLC and UHPLC, you can apply the same information for the development of UHPLC methods. Here’s what the course covers:
- What Quality by Design (QbD) is and how it applies to HPLC methods
- A review of basic HPLC theory as it applies to method development
- The makeup of HPLC columns including the support material and the bonded phase
- The role of solvents in the separation
- How the column plate number, selectivity, and retention can be used in a logical way to solve even the
toughest separation problems.
- How to use mobile phase solvents, pH, temperature, chemistry, and other variables to pull apart
difficult-to-separate peak pairs.
- How gradient elution works and how to remove the mystery of optimizing gradients.
- How a scouting gradient can be used to speed the development of both isocratic and gradient methods.
- How to use a simple spreadsheet calculator to move from the scouting run to the next step in method
- How computer-assisted method development tools can leverage a small amount of data into a large amount of information about a separation
- How to take advantage of a publicly available internet database to identify equivalent and orthogonal columns
- When standard reversed-phase techniques don’t work, how to choose mixed-mode, ion-pairing, normal phase, HILIC, chiral, or other chromatographic modes
- What’s the same and different when comparing HPLC and UHPLC
- What to look for when validating a method
Presenter: John Dolan
John Dolan is considered to be the one of the world’s experts in HPLC. He has written more than 300 user-oriented articles on HPLC troubleshooting over the last 30 years in addition to more than 100 peer-reviewed technical articles on HPLC and related techniques. His three books (co-authored with Lloyd Snyder), Troubleshooting HPLC Systems, Introduction to Modern Liquid Chromatography (3rd edn), and High-Performance Gradient Elution, are standard references on thousands of desks around the world. He has taught HPLC training classes around the world to more than 10,000 students.