Fundamentals of HPLC

‘Fundamentals of HPLC’ covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation, including:

  • The basics of the chromatographic process
  • Terms and definitions of chromatography
  • How HPLC systems work
  • Practical tips on preventive maintenance
  • Principles of separation chemistry
  • Why “reversed phase” is the standard separation technique
  • The meaning of “gradients” and why they are used
  • How to get the best quantitation
  • and much more

By taking this online course you will acquire a good understanding of the fundamentals of HPLC and get a better knowledge of the key concepts involved. The course is aimed at managers, supervisors, auditors, reviewers, etc. who use information generated by HPLC but do not necessarily do chromatography themselves.

Presenter: Tom Jupille

Tom has been a practising chromatographer for more than 45 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in late ‘70s. His career has focused on instrument and column development and user support, providing a broad foundation of practical experience to call on as an instructor. He is moderator of the popular Chromatography Forum on-line chromatography discussion group.

For a better understanding of the course format, view the first module below:



Take this Course