Practical HPLC for Biopharmaceuticals
Although “small molecule” and “biopolymer” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with both. Fortunately, the underlying principles of chromatography apply equally well in both situations when interpreted appropriately.
“Practical HPLC for Biopharmaceuticals” explains chromatography in practical terms from the ground up. Here’s what you’ll learn in this online course:
- The basics of isocratic and gradient HPLC
- Reversed phase and ion exchange HPLC
- HPLC equipment – from reservoirs/pumps to tubing/injectors to columns and detectors
- Understanding and overcoming common column problems
- Alternative modes: normal phase, HILIC and size exclusion
- Performance qualification
- Troubleshooting and diagnostics
You’ll find out the various roles that the different separation techniques play in the determination, characterization and quantitation of both small molecules and biopolymers. As well as conventional reversed phase HPLC you’ll will learn about ion-exchange, HILIC, size-exclusion chromatography and hydrophobic interaction chromatography – providing you with an analytical toolbox to apply to your own analytical challenges.